1. Explain the composition and function of euchromatin. How does the chromatin state affect gene expression? How does chromatin remodelling work in eukaryotic cells? Explain a technique to identify chromatin states in a specific cell or tissue. /4
2. Explain the various roles of ncRNA in eukaryotes. /4
3. Explain TP53 in detail. /3
4. Explain following terms: /5
   • PRC
   • Imprinting
   • Epitranscriptomics
   • Flowering locus C
   • Histone methylation

• Transcription factors: functie, domeinen, met wat interageren, hoe interactiepartners determineren?
• Welke non-coding RNAs en functies
• Hoe wordt de flowering van Arabidopsis epigenetisch gereguleerd?

Kleine vraagjes: leg uit in een paar lijnen

• TP53
• PRC
• Poised genes
• 2 anderen die ik vergeten ben

3 Big questions:

1. Why are transcription factors important for eukaryotic gene expression? What are their different domains? What are the major types? (2/20)
2. To what do transcription factors bind and how can you detect this? (2/20)
3. Explain what histone modifications are and how do they influence gene expression (4/20)
4. What is the role of non-coding RNAs in gene expression regulation? (3/20)

Describe in only a few lines (1/20 each)

• P53
• Flowering locus C
• PcG complexes
• Chromatin remodeling
• X chromosome inactivation

1. Why are transcription factors important for eukaryotic gene expression? What are their different domains? What are the major types? Describe how you can search where they specifically bind. (4/20)
2. Explain genetic imprinting. Describe an example and how imprinting is regulated. (4/20)
3. What is chromatin remodeling and how does it play a role in eukaryotic gene expression? (3/20)
4. Describe in maximum 3 lines (each 1/20, total 5/20):
   • PRC complex
   • Bisulfite treatment
   • miRNA
   • RdDM pathway
   • Histone acetylation

+ task 4/20 in advance